Veterinary Diagnostic User Fees
APHIS laboratory services are provided by the National Veterinary Services Laboratories (NVSL) in Ames, IA, and Orient Point (Plum Island), NY. The NVSL provides a wide variety of information and services, centered around diagnosis of domestic and foreign animal diseases, support of disease control and eradication programs, reagents for diagnostic testing, training, and laboratory certification.
National Veterinary Services Laboratories (NVSL) Detailed Information
Code of Federal Regulations Part 130.1-130.7
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- 9CFR 130: Foreign Animal Disease Diagnostics Laboratory (FADDL) Veterinary Diagnostics
- 9CFR 130: Certain Veterinary Diagnostic Tests Performed at FADDL
- 9CFR 130: Other Veterinary Diagnostic Services Performed at FADDL
- 9CFR 130: Veterinary Diagnostic Isolation and Identification Tests Performed at NVSL (Excluding FADDL) or Other Authorized Site
- 9CFR 130: Veterinary Diagnostic Serology Tests Performed at NVSL or at Authorized Sites
- 9CFR 130: Virology Serology Tests Performed at NVSL (Excluding FADDL) or at Authorized Site
- 9CFR 130: Other Veterinary Diagnostics Laboratory Tests Performed at NVSL (Excluding FADDL) or at Authorized Sites
- 9CFR 130: Veterinary Diagnostic Reagents Produced at NVSL or Other Authorized Sites
- 9CFR 130: Virology Reagents
- 9CFR 130: Other Veterinary Diagnostic Services or Materials Provided at NVSL (Excluding FADDL)
§130—User Fees for Foreign Animal Disease Diagnostics Laboratory (FADDL) Veterinary Diagnostics
APHIS charges user fees for diagnostic reagents1 provided by FADDL. Prior to the August 2023 final rule, these fees were set forth in § 130.14(a) of the regulations.
Reagent | Unit | User Fee |
|---|---|---|
| Bovine antiserum, any agent | 1 mL | $334.00 |
| Caprine antiserum, any agent | 1 mL | 409.00 |
| Cell culture antigen/microorganism | 1 mL | 225.00 |
| Equine antiserum, any agent | 1 mL | 413.00 |
| Fluorescent antibody conjugate | 1 mL | 363.00 |
| Guinea pig antiserum, any agent | 1 mL | 405.00 |
| Monoclonal antibody | 1 mL | 492.00 |
| Ovine antiserum, any agent | 1 mL | 391.00 |
| Porcine antiserum, any agent | 1 mL | 338.00 |
| Rabbit antiserum, any agent | 1 mL | 397.00 |
§130—User Fees for Certain Veterinary Diagnostic Tests Performed at FADDL
APHIS charges user fees for veterinary diagnostic tests performed at FADDL. Prior to the August 2023 final rule, these fees were set forth in paragraph (b) of § 130.14.
Test | Unit | User Fee |
|---|---|---|
| Agar gel immunodiffusion | Test | $67.00 |
| Card | Test | 37.00 |
| Complement fixation | Test | 81.00 |
| Direct immunofluorescent antibody | Test | 51.00 |
| Enzyme linked immunosorbent assay | Test | 60.00 |
| Fluorescent antibody neutralization (classical swine fever) | Test | 436.00 |
| Hemagglutination inhibition | Test | 128.00 |
| Immunoperoxidase. | Test | 65.00 |
| Indirect fluorescent antibody | Test | 79.00 |
| In-vitro safety | Test | 618.00 |
| In-vivo safety | Test | 11,147.00 |
| Latex agglutination. | Test | 53.00 |
| Tube agglutination. | Test | 61.00 |
| Virus isolation (oesophageal/pharyngeal) | Test | 403.00 |
| Virus isolation in embryonated eggs | Test | 775.00 |
| Virus isolation, other | Test | 178.00 |
| Virus neutralization | Test | 118.00 |
§130—User Fees for Other Veterinary Diagnostic Services Performed at FADDL
APHIS charges user fees for other veterinary diagnostic tests performed at FADDL. Prior to the August 2023 final rule, these fees were set forth in paragraph (c) of § 130.14.
Veterinary diagnostic service | Unit | User Fee |
|---|---|---|
| Bacterial isolation | Test | $249.00 |
| Hourly user fee services2 | Hour | 996.00 |
| Hourly user fee services—Quarter hour | Quarter hour | 249.00 |
| Infected cells on chamber slides or plates | Slide | 108.00 |
| Reference animal tissues for immunohistochemistry | Set | 379.00 |
| Sterilization by gamma radiation | Can | 3,891.00 |
| Training (school or technical assistance) | Per person per day | 2,036.00 |
| Virus titration | Test | 249.00 |
2For all veterinary diagnostic services for which there is no flat rate user fee, the hourly rate user fee will be calculated for the actual time re- quired to provide the service. | ||
§130—User Fees for Veterinary Diagnostic Isolation and Identification Tests Performed at NVSL (Excluding FADDL) or Other Authorized Site
APHIS charges user fees for veterinary diagnostic isolation and identification tests performed at NVSL (excluding FADDL) or other authorized sites. Prior to the August 2023 final rule, these fees were set forth in § 130.15 paragraph (a) of the regulations.
Test | Unit | User Fee |
|---|---|---|
| Bacterial identification, automated | Isolate | $124.00 |
| Bacterial identification, non-automated | Isolate | 199.00 |
| Bacterial isolation | Sample | 81.00 |
| Bacterial serotyping, all other | Isolate | 118.00 |
| Bacterial serotyping, Pasteurella multocida | Isolate | 42.00 |
| Bacterial serotyping, Salmonella | Isolate | 97.00 |
| Bacterial toxin typing | Isolate | 404.00 |
| Bacteriology requiring special characterization | Test | 215.00 |
| DNA fingerprinting | Test | 430.00 |
| DNA probe | Test | 181.00 |
| Fluorescent antibody | Test | 42.00 |
| Mycobacterium identification (biochemical) | Isolate | 253.00 |
| Mycobacterium identification (gas chromatography) | Procedure | 213.00 |
| Mycobacterium isolation, animal inoculations | Submission | 1,822.00 |
| Mycobacterium isolation, all other | Submission | 334.00 |
| Mycobacterium paratuberculosis isolation | Submission | 160.00 |
| Phage typing, all other | Isolate | 94.00 |
| Phage typing, Salmonella enteritidis | Isolate | 53.00 |
§130 —User Fees for Virology Identification Tests Performed at NVSL (Excluding FADDL) or Other Authorized Site
APHIS charges user fees for virology identification tests performed at NVSL (excluding FADDL) or other authorized sites. Prior to the August 2023 final rule, these fees were also set forth in § 130.15 paragraph (b) of the regulations.
Test | Unit | User Fees |
|---|---|---|
| Fluorescent antibody tissue section | Test | $63.00 |
| Virus isolation | Test | 108.00 |
§130—User Fees for Veterinary Diagnostic Serology Tests Performed at NVSL or at Authorized
APHIS charges user fees for veterinary diagnostic serology tests performed at NVSL or at authorized sites. Prior to the August 2023 final rule, these fees were set forth in § 130.16 paragraph (a) of the regulations.
Test | Unit | User Fees |
|---|---|---|
| Brucella ring (BRT) | Test | $84.00 |
| Brucella ring, heat inactivated (HIRT) | Test | 81.00 |
| Brucella ring, serial (Serial BRT) | Test | 120.00 |
| Buffered acidified plate antigen presumptive | Test | 17.00 |
| Card | Test | 8.75 |
| Complement fixation | Test | 52.00 |
| Enzyme linked immunosorbent assay | Test | 49.00 |
| Indirect fluorescent antibody | Test | 52.00 |
| Microscopic agglutination—includes up to 5 serovars | Sample | 60.00 |
| Microscopic agglutination—each serovar in excess of 5 serovars | Sample | 9.75 |
| Particle concentration fluorescent immunoassay (PCFIA) | Test | 79.00 |
| Plate | Test | 17.00 |
| Rapid automated presumptive | Test | 15.00 |
| Rivanol | Test | 16.00 |
| Tube agglutination | Test | 17.00 |
§130 —User Fees for Virology Serology Tests Performed at NVSL (Excluding FADDL) or at Authorized Sites
APHIS charges user fees for virology serology tests performed at NVSL (excluding FADDL) or at authorized sites. Prior to the August 2023 final rule, these fees were also set forth in § 130.16 paragraph (b) of the regulations.
Test | Unit | User Fee |
|---|---|---|
| Agar gel immunodiffusion | Test | $33.00 |
| Complement fixation | Test | 52.00 |
| Enzyme linked immunosorbent assay | Test | 49.00 |
| Hemagglutination inhibition | Test | 52.00 |
| Indirect fluorescent antibody | Test | 52.00 |
| Latex agglutination | Test | 37.00 |
| Peroxidase linked antibody | Test | 35.00 |
| Plaque reduction neutralization | Test | 54.00 |
| Rabies fluorescent antibody neutralization | Test | 102.00 |
| Virus neutralization | Test | 52.00 |
§130—User Fees for Other Veterinary Diagnostics Laboratory Tests Performed at NVSL (Excluding FADDL) or at Authorized Sites
APHIS charges user fees for virology serology tests performed at NVSL (excluding FADDL) or at authorized sites. Prior to the August 2023 final rule, these fees were also set forth in § 130.16 paragraph (b) of the regulations.
Test | Unit | User Fee |
|---|---|---|
| Aflatoxin quantitation | Test | $67.00 |
| Aflatoxin screen | Test | 63.00 |
| Agar gel immunodiffusion spp. identification | Test | 29.00 |
| Antibiotic (bioautography) quantitation | Test | 146.00 |
| Antibiotic (bioautography) screen | Test | 264.00 |
| Antibiotic inhibition | Test | 146.00 |
| Arsenic | Test | 39.00 |
| Ergot alkaloid screen | Test | 146.00 |
| Ergot alkaloid confirmation | Test | 191.00 |
| Feed microscopy | Test | 146.00 |
| Fumonisin only | Test | 81.00 |
| Gossypol | Test | 217.00 |
| Mercury | Test | 320.00 |
| Metals screen | Test | 98.00 |
| Metals single element confirmation | Test | 29.00 |
| Mycotoxin: aflatoxin-liver | Test | 264.00 |
| Mycotoxin screen | Test | 106.00 |
| Nitrate/nitrite | Test | 146.00 |
| Organic compound confirmation | Test | 195.00 |
| Organic compound screen | Test | 334.00 |
| Parasitology | Test | 60.00 |
| Pesticide quantitation | Test | 292.00 |
| Pesticide screen | Test | 134.00 |
| pH | Test | 59.00 |
| Plate cylinder | Test | 217.00 |
| Selenium | Test | 98.00 |
| Silicate/carbonate disinfectant | Test | 146.00 |
| Temperature disks | Test | 288.00 |
| Toxicant quantitation, other | Test | 243.00 |
| Toxicant screen, other | Test | 73.00 |
| Vomitoxin only | Test | 118.00 |
| Water activity | Test | 73.00 |
| Zearaleone quantitation | Test | 118.00 |
| Zearaleone screen | Test | 63.00 |
§130—User Fees for Veterinary Diagnostic Reagents Produced at NVSL or Other Authorized Sites
APHIS charges user fees for bacteriology reagents produced by the Diagnostic Bacteriology Laboratory at NVSL (excluding FADDL) or other authorized sites. Prior to the August 2023 final rule, these fees were set forth in § 130.18 paragraph (a) of the regulations.
Reagent | Unit | User Fee |
|---|---|---|
| Anaplasma card test antigen | 2 mL | $209.00 |
| Anaplasma card test kit without antigen | Kit | 282.00 |
| Anaplasma CF antigen | 2 mL | 96.00 |
| Anaplasma stabilate | 4.5 mL | 404.00 |
| Avian origin bacterial antiserums | 1 mL | 215.00 |
| Bacterial agglutinating antigens other than brucella and salmonella pullorum | 5 mL | 140.00 |
| Bacterial conjugates | 1 mL | 108.00 |
| Bacterial disease CF antigens, all other | 1 mL | 65.00 |
| Bacterial ELISA antigens | 1 mL | 65.00 |
| Bacterial or protozoal antiserums, all other | 1 mL | 146.00 |
| Bacterial reagent culture1 | Culture | 242.00 |
| Bacterial reference culture2 | Culture | 645.00 |
| Bacteriophage reference culture | Culture | 381.00 |
| Bovine serum factor | 1 mL | 39.00 |
| Brucella abortus CF antigen | 60 mL | 350.00 |
| Brucella agglutination antigens, all other | 60 mL | 334.00 |
| Brucella buffered plate antigen | 60 mL | 404.00 |
| Brucella canis tube antigen | 25 mL | 264.00 |
| Brucella card test antigen (packaged) | Package | 199.00 |
| Brucella card test kit without antigen | Kit | 248.00 |
| Brucella cells | Gram | 41.00 |
| Brucella cells, dried | Pellet | 13.00 |
| Brucella ring test antigen | 60 mL | 527.00 |
| Brucella rivanol solution | 60 mL | 65.00 |
| Dourine CF antigen | 1 mL | 597.00 |
| Dourine stabilate | 4.5 mL | 235.00 |
| Equine and bovine origin babesia species antiserums | 1 mL | 334.00 |
| Equine negative control CF antigen | 1 mL | 593.00 |
| Flazo-orange | 3 mL | 29.00 |
| Glanders CF antigen | 1 mL | 170.00 |
| Hemoparasitic disease CF antigens, all other | 1 mL | 1,194.00 |
| Leptospira transport medium | 10 mL | 9.75 |
| Monoclonal antibody | 1 mL | 209.00 |
| Mycobacterium spp. old tuberculin | 1 mL | 53.00 |
| Mycobacterium spp. PPD | 1 mL | 41.00 |
| Mycoplasma hemagglutination antigens | 5 mL | 430.00 |
| Negative control serums | 1 mL | 43.00 |
| Rabbit origin bacterial antiserum | 1 mL | 114.00 |
| Salmonella pullorum microagglutination antigen | 5 mL | 35.00 |
| Stabilates, all other | 4.5 mL | 1,478.00 |
1 A reagent culture is a bacterial culture that has been subcultured one or more times after being tested for purity and identity. It is intended for use as a reagent with a diagnostic test such as the leptospiral agglutination test. 2 A reference culture is a bacterial culture that has been thoroughly tested for purity and identity. It should be suitable as a master seed for future cultures. | ||
§130—User Fees for Virology Reagents
APHIS charges user fees for virology reagents produced by the Diagnostic Virology Laboratory at NVSL (excluding FADDL) or at authorized sites. Prior to the August 2023 final rule, these fees were set forth in § 130.18 paragraph (b) of the regulations.
Reagent | Unit | User Fee |
|---|---|---|
| Antigen, except avian influenza and chlamydia psittaci antigens, any | 2 mL | $140.00 |
| Avian antiserum except avian influenza antiserum, any | 2 mL | 135.00 |
| Avian influenza antigen, any | 2 mL | 76.00 |
| Avian influenza antiserum, any | 6 mL | 242.00 |
| Bovine or ovine serum, any | 2 mL | 280.00 |
| Cell culture | Flask | 683.00 |
| Chlamydia psittaci spp. of origin monoclonal antibody panel | Panel | 205.00 |
| Conjugate, any | 1 mL | 167.00 |
| Diluted positive control serum, any | 2 mL | 57.00 |
| Equine antiserum, any | 2 mL | 103.00 |
| Monoclonal antibody | 1 mL | 242.00 |
| Other spp. antiserum, any | 1 mL | 108.00 |
| Porcine antiserum, any | 2 mL | 242.00 |
| Porcine tissue sets | Tissue set | 326.00 |
| Positive control tissues, all | 2 cm2 section | 134.00 |
| Rabbit origin antiserum | 1 mL | 114.00 |
| Reference virus, any | 0.6 mL | 645.00 |
| Viruses (except reference viruses), chlamydia psittaci agent or chlamydia psittaci anti- gen, any | 0.6 mL | 70.00 |
§130—User Fees for Other Veterinary Diagnostic Services or Materials Provided at NVSL (Excluding FADDL)
APHIS charges user fees for other veterinary diagnostic services or materials available from NVSL (excluding FADDL). Prior to the August 2023 final rule, these fees were set forth in § 130.19 paragraph (a) of the regulations.
Service | Unit | User Fee |
|---|---|---|
| Antimicrobial susceptibility test | Isolate | $242.00 |
| Avian safety test | Test | 8,636.00 |
| Check tests, culture | Kit 1 | 774.00 |
| Check tests, serology | Kit 1 | 774.00 |
| Fetal bovine serum safety test | Verification | 2,419.00 |
Hourly user fees2 | ||
| Hour | Hour | $143.00 |
| Quarter hour | Quarter Hour | 36.00 |
| Minimum | 43.00 | |
| Manual, brucellosis culture | 1 copy | 253.00 |
| Manual, tuberculosis Culture (English or Spanish) | 1 copy | 381.00 |
| Manual, Veterinary mycology | 1 copy | 381.00 |
| Manuals or standard operating procedure (SOP), all other | 1 copy | 75.00 |
| Manuals or SOP, per page | 1 page | 5.75 |
| Training (school or technical assistance) | Per person per day | 753.00 |
1Any reagents required for the check test will be charged separately. 2 For veterinary diagnostic services for which there is no flat user fee the Hourly rate user fee will be calculated for the actual time required to provide the service. | ||