Supplemental Assay Methods (SAMs)

Last Modified: June 27, 2024
IDTitleDate
101Titration of Bovine Viral Diarrhea Virus in Vaccines [Withdrawn]--
102Titration of Parainfluenza-3 Virus in Vaccines [Withdrawn]--
104 (146.07 KB)Isolation of Parainfluenza-3 Virus from Nasal ExcretionsOctober 10, 2014
105Titration of Bovine Rhinotracheitis Virus in Vaccines [Withdrawn]--
106 (181.85 KB)Titration of Neutralizing Antibody (Constant Virus - Varying Serum Method)October 16, 2014
107 (164.92 KB)Titration of Neutralizing Antibody (Constant Serum - Varying Virus Method)October 16, 2014
108 (189.22 KB)Detection of Extraneous Bovine Viral Diarrhea Virus in Modified-Live VaccinesOctober 16, 2014
109 (295.5 KB)Titration of Bovine Rhinotracheitis Neutralizing Antibody (Constant Virus - Varying Serum Method)October 16, 2014
110 (257.13 KB)Titration of Eastern, Western, and Venezuelan Equine Encephalomyelitis Virus Neutralizing AntibodiesApril 05, 2016
111Titration of Infectious Bovine Rhinotracheitis Virus Neutralizing Antibody (Constant Virus - Varying Serum Method) [Withdrawn]--
112 (255.58 KB)Titration of Bovine Viral Diarrhea Neutralizing Antibody (Constant Virus - Varying Serum Method)October 16, 2014
113 (278.25 KB)Titration of Parainfluenza-3 Neutralizing Antibody (Constant Virus - Varying Serum Method)May 12, 2017
114 (191.01 KB)Titration of Porcine Transmissible Gastroenteritis VirusOctober 16, 2014
115 (183.31 KB)Titration of Bovine Viral Diarrhea Neutralizing Antibody (Constant Serum - Varying Virus Method)October 16, 2014
116 (158.05 KB)Phenotypic Examination of Pseudorabies Virus for Thymidine Kinase Activity by a Plaque Selection MethodOctober 16, 2014
117 (254.5 KB)Titration of Pseudorabies Virus Neutralizing Antibody (Constant Virus - Varying Serum Methods)October 16, 2014
118 (130.5 KB)Titration of Pseudorabies Virus in VaccinesAugust 7, 2023
119 (172.12 KB)Titration of Pseudorabies Virus Neutralizing Antibody (Constant Virus - Varying Serum Method)August 7, 2023
120 (170.47 KB)In Vitro Potency Assay of Bovine Respiratory Viruses in Killed VaccinesOctober 16, 2014
121 (148.42 KB)Titration of Porcine Rotavirus in Modified-live VaccinesOctober 21, 2014
122 (216.34 KB)Titration of Porcine Rotavirus Antibody (Constant Virus-Varying Serum Method)October 21, 2014
123 (171.34 KB)Pseudorabies Virus Challenge Test in SwineOctober 21, 2014
124 (226.66 KB)Conducting the Hemagglutination Inhibition Assay for Equine Influenza AntibodyOctober 23, 2014
126 (185.52 KB)Titration of Bovine Rotavirus in Vaccines (Constant Virus - Varying Serum Method)October 23, 2014
IDTitleDate
200 (156.86 KB)Potency Testing Products Containing Clostridium chauvoei AntigenDecember 13, 2017
201 (163.69 KB)Potency Testing Clostridium perfringens Type C Beta AntigenAugust 24, 2023
202Potency Testing Clostridium perfringens Type C Beta AntitoxinsAugust 24, 2023
203 (166.81 KB)Potency Testing Clostridium perfringens Type D Epsilon AntigenAugust 24, 2023
204Potency Testing Clostridium perfringens Type D Epsilon AntitoxinsSeptember 7, 2023
206 (203.06 KB)Potency Testing Tetanus AntitoxinsDecember 18, 2017
207 (275.62 KB)Potency Testing Clostridium novyi Type B Alpha AntigenAugust 10, 2023
209 (157.03 KB)Potency Testing Products Containing Clostridium haemolyticum AntigenDecember 13, 2017
212 (166.54 KB)Potency Testing Clostridium sordellii AntigenAugust 10, 2023
213 (158.45 KB)Potency Testing of Clostridium botulinum Type C Bacterin-ToxoidsJune 17, 2022
214 (155.75 KB)Immunogenicity and Spore Count Testing of Anthrax Spore Vaccine-NonencapsulatedJune 12, 2022
217 (228.44 KB)Potency Testing of Tetanus Toxoid by ELISAJune 17, 2022
IDTitleDate
300 (185.81 KB)
Titration of Neutralizing Antibody - Canine HepatitisAugust 22, 2023
301 (211.26 KB)Titration of Neutralizing Antibody - Canine DistemperAugust 22, 2023
302 (212.99 KB)Titration of Canine Distemper Virus in Primary Cell CultureOctober 29, 2014
303 (206.91 KB)Titration of Distemper Virus in Embryonated Chicken EggsOctober 30, 2014
304 (205.85 KB)Titration of Infectious Canine Hepatitis Virus in Primary Canine Kidney Cell CultureOctober 30, 2014
305 (208.69 KB)Titration of Feline Panleukopenia Virus in Cell CultureOctober  30, 2014
306 (179.35 KB)Titration of Feline Calicivirus in Cell CultureOctober  30, 2014
307 (155.38 KB)Titration of Feline Rhinotracheitis Virus in Cell CultureOctober 30, 2014
308 (176.85 KB)Potency Testing of Inactivated Rabies Vaccine in Mice Using the National Institutes of Health TestMarch 31, 2015
309 (215.01 KB)Titration of Canine Parainfluenza Virus in Vero Cell CultureOctober  30, 2014
310Scoring Feline Calicivirus in Cats Following Challenge [Withdrawn]--
311Scoring Feline Rhinotracheitis Virus in Cats Following Challenge [Withdrawn]--
312 (124.13 KB)Detection of Extraneous Cytopathogenic Agents in Master Seed VirusOctober  30, 2014
313 (272.38 KB)Detection of Extraneous Hemadsorbing Agents in Master Seed VirusAugust 23, 2023
314 (150.1 KB)Detection of Extraneous Viruses by the Fluorescent Antibody TestNovember 13, 2014
315 (181 KB)Detection of Serum Neutralizing Antibodies to Rabies Virus using the Rapid Fluorescent Focus Inhibition Test (RFFIT)November 13, 2014
316 (205.34 KB)Titration of Canine Parvovirus in Cell CultureNovember 14, 2014
317 (200.82 KB)Titration of Canine Adenovirus in Canine Kidney Cell CultureNovember 13, 2014
318Estimating Relative Potency by the Automated Selection of Line Segments in RelPot 4.0 Software [Withdrawn]--
319 (307 KB)Titration of Chlamydophila felis (formerly Feline Chlamydia psittaci) in Cell CultureNovember 13, 2014
320 (144.95 KB)Titration of Feline Chlamydia psittaci in Embryonated Chicken EggsNovember 13, 2020
321 (164.72 KB)Quantitating the GP70 Antigen of Feline Leukemia Virus in Veterinary VaccinesJanuary 9, 2015
322 (213.13 KB)Determination of the Specific Viral Antigen Content in Inactivated Canine Coronavirus VaccinesJanuary 9, 2015
323 (205.35 KB)Titration of Canine Distemper Virus in Vero Cell CultureNovember 20, 2020
IDTitleDate
405 (412.95 KB)Detecting Lymphoid Leukosis Biocontamination by the COFAL TestNovember 28, 2014
406 (234 KB)Titration of Monovalent, Cell Associated Marek′s Disease Vaccines of Serotypes 1, 2 or 3November 20, 2014
407 (218.48 KB)Titration of Marek's Serotype 3 (Herpesvirus of Turkeys Strain FC-126), LyophilizedNovember 20, 2014
408 (158.28 KB)Titrating Tissue Culture Adapted Vaccine Strain of Infectious Bursal Disease VirusNovember 28, 2014
409 (122.5 KB)Titrating the Fractions of Combination Avian Encephalomyelitis/Pox VaccineNovember 28, 2014
411 (157.13 KB)Titration of Newcastle Disease Vaccine, Infectious Bronchitis Vaccine, and Combination Newcastle Disease-Infectious Bronchitis Vaccine in Chicken EmbryosJanuary 10, 2018
IDTitleDate
500 (1.01 MB)Determination of Phenol in Veterinary Biologics (Pullorum antigen, Mycoplasma synoviae antigen, and Mycoplasma gallisepticum antigen)June 16, 2022
502 (1.69 MB)Determination of Residual Moisture in Veterinary Biologics ProductsJune 17, 2022
504 (882.54 KB)Manual Determination of Protein Content of Veterinary Biologics (Biuret)June 17, 2022
509Mikrozone Electrophoretic Separation of Blood Fractions Used in Veterinary Biologics [Withdrawn]--
512 (162.32 KB)Determination of Formaldehyde in Veterinary Biologics (Ferric Chloride Test)April 5, 2022
513Determination of Protein and Phenol in PPD Bovis Tuberculin (Purified Protein Derivative Produced from Cultures of Mycobacterium bovis (Strain AN-5))January 5, 2024
514Determination of Hydrogen Ion Concentration, Total Nitrogen, Phenol and Clarity in Intradermic Tuberculin (Filtrate Produced From Cultures of Pn, C, and Dt Strains of Mycobacterium tuberculosis)January 5, 2024
IDTitleDate
600 (224.18 KB)Purity, Potency, and Dissociation of Brucella abortus Vaccine, Strain 19January 3, 2022
601Potency Testing of Erysipelas Antiserum in Mice [Withdrawn]--
602 (46.36 KB)Evaluation of Salmonella Pullorum AntigensJanuary 28, 2010
603 (925.05 KB)Potency Testing of Fowl Cholera (Pasteurella multocida) Bacterins, Type 3June 17, 2022
604 (1.09 MB)Evaluation of Koch's Old TuberculinJune 17, 2022
605Potency Testing of Erysipelas Bacterins in Swine [Withdrawn]--
606Potency Testing of Erysipelas Vaccines in Swine [Withdrawn]--
607 (929.34 KB)Potency Testing of Fowl Cholera (Pasteurella multocida) Bacterins, Type 1June 17, 2022
608 (257.19 KB)Potency Assay of Leptospira interrogans serogroup pomona BacterinsDecember 23, 2022
609 (254.96 KB)Potency Assay of Leptospira interrogans serogroup canicola BacterinsApril 19, 2022
610 (242.52 KB)Potency Assay of Leptospira interrogans serogroup icterohaemorrhagiae BacterinsDecember 23, 2022
611 (221.17 KB)Potency Testing of Erysipelas Bacterins in MiceDecember 15, 2017
612 (126.2 KB)Bacterial Plate Count of Erysipelothrix rhusiopathiae VaccinesFebruary 05, 2015
613 (906.22 KB)In vitro Potency Testing of Erysipelothrix rhusiopathiae BacterinsJune 17, 2022
617 (225.91 KB)Potency Assay Leptospira kirschneri serogroup grippotyphosa BacterinsApril 26, 2022
620 (256.57 KB)Potency Testing of Enterotoxigenic (K99 Pilus) Escherichia coli BacterinsMay 26, 2023
621 (245.17 KB)Potency Testing of Enterotoxigenic (K88 Pilus) Escherichia coli BacterinsMay 26, 2023
622 (246.24 KB)Potency Testing of Enterotoxigenic (987P Pilus) Escherichia coli BacterinsMay 26, 2023
623 (248.28 KB)Potency Testing Enterotoxigenic (F41 Pilus) Escherichia coli BacterinsMay 26, 2023
624 (215.31 KB)In vitro Potency Testing of Leptospira interrogans serogroup pomona BacterinsMay 28, 2023
625 (229.39 KB)In vitro Potency Testing of Leptospira interrogans serogroup canicola BacterinsMay 28, 2023
626 (216.91 KB)In vitro Potency Testing of Leptospira kirschneri serogroup grippotyphosa BacterinsMay 28, 2023
627 (233.25 KB)In vitro Potency Testing of Leptospira interrogans serogroup icterohaemorrhagiae BacterinsMay 28, 2023
630 (924.18 KB)Potency Testing of Fowl Cholera (Pasteurella multocida) Bacterins, Type 4June 17, 2022
631 (933.65 KB)Potency Testing of Salmonella typhimurium BacterinsJune 17, 2022
632 (936.52 KB)Potency Testing of Salmonella choler aesuis BacterinsJune 17, 2022
633 (968.02 KB)Potency Testing of Salmonella dublin BacterinsJune 17, 2022
634 (172.1 KB)Potency Testing of Pasteurella multocida Bacterins of Bovine OriginDecember 18, 2017
635 (217.46 KB)Potency Testing of Pasteurella multocida Bacterins of Porcine OriginDecember 18, 2017
636 (1.01 MB)Evaluation of Tuberculin Purified Protein Derivative (PPD)June 17, 2022
IDTitleDate
801 (160.39 KB)Examination of Porcine Cells for Porcine Parvovirus ContaminationNovember 28, 2014
803 (159.05 KB)Examination of Trypsin Solutions for Porcine Parvovirus ContaminationNovember 28, 2014
IDTitleDate
900Testing Growth Promoting Qualities of Fluid Thioglycollate Medium and Soybean-Casein Digest Medium using Bacillus subtilis Spores and Issatchenkia orientalis as the Indicator OrganismsJanuary 5, 2024
901Testing Growth-Promoting Qualities of Fluid Thioglycollate Medium with Beef Extract Using Clostridium chauvoei Spores as the Indicator OrganismJanuary 5, 2024
902Testing Growth-Promoting Qualities of Brain Heart Infusion Agar using Bacillus subtilis Spores and Issatchenkia orientalis as Indicator OrganismsJanuary 5, 2024
903 (210.16 KB)Testing for Preservative Interference with Sterility TestsJanuary 3, 2022
905 (227.51 KB)Potency of Live Avirulent Mannheimia haemolytica VaccineJanuary 3, 2022
906 (256.34 KB)Sterility Testing of Preparations Other Than Live VaccinesMay 12, 2023
908 (252.27 KB)Sterility Testing of Live Viral Vaccines and Master Seed Virus SamplesMay 12, 2023
909 (230.64 KB)Sterility Testing of Live Viral Vaccines of Chicken Embryo Origin Recommended for Nonparenteral InjectionMay 12, 2023
910 (248.48 KB)Detection of Mycoplasma ContaminationFebruary 21, 2023
911 (250.03 KB)Detection of Salmonella in Antibody ProductsApril 27, 2023
912 (203.68 KB)Determination of Total Bacterial Count in Antibody ProductsFebruary 21, 2023
913 (230.76 KB)Detection of Coliforms in Antibody ProductsApril 27, 2023
914 (193.99 KB)Detection of Fungi in Antibody ProductsMay 15, 2023
918 (212.26 KB)Bacterial Count of Pasteurella multocida, Avian Isolates, VaccinesFebruary 21, 2023
928 (227.99 KB)Detection of Extraneous Bacteria and Fungi in Live Bacterial Vaccines and Master Seed Bacteria SamplesJuly 26, 2018