APHIS eFile Permitting Resources and Training

Last Modified: April 08, 2026

General eFile Materials

For general guidance related to APHIS eFile, please reference the following:

APHIS eFile First Time User Guide (1.45 MB)
APHIS eFile Team Sharing Accounts (701.91 KB)
APHIS eFile Overview (925.06 KB)

Watch our APHIS eFile Account Registration and Management video on YouTube

Animal Care Live Dogs

For guidance specific to importing live dogs into the Continental United States, please reference the following :

New Guidance for Requesting a Permit to Import Live Dogs (1.67 MB)

Watch our new Live Dog Import "How To" Videos on YouTube

Biotechnology Regulatory Services

For guidance specific to BRS applications in APHIS eFile, please reference the following documents:

Permit User Guide (972.79 KB)
Notification User Guide (727.27 KB)
APHIS 2000 Authorization Application and Compliance Reporting Job Aid (8.95 MB)
SOP Template for APHIS BRS Plant Permits
SOP Template for APHIS BRS Modified Microbe Movement Permits (261.35 KB)
BRS Applicant Training Slides and Videos (3.24 MB) (April 2022)

Plant Protection and Quarantine

PPQ 525A

For guidance on PPQ 525A requests for permits to receive soil for non-biological uses, please reference the following:

Guidance for Submitting a PPQ 525A Permit Application (3.04 MB)

Watch our PPQ 525A “How To” videos on YouTube

PPQ 526

For guidance on PPQ 526 requests for permits to move live pests, noxious weeds, and soil for biological uses, please reference the following:

Guidance for Submitting a PPQ 526 Permit Application (2.87 MB)

Watch our PPQ 526 “How To” videos on YouTube

PPQ 546

For guidance on PPQ 546 request for Postentry Quarantine agreements, please reference the following:

Guidance for Submitting a PPQ 546 Application for Postentry Quarantine Agreement (2.5 MB)

Watch our PPQ 546 "How To" videos on YouTube

PPQ 585

For guidance on PPQ 585 requests for Timber and Timber Products permits, please reference the following:

Guidance for Submitting a PPQ 585 Permit Application (2.13 MB)

Watch our PPQ 585 "How To" videos on YouTube

PPQ 586

For guidance on PPQ 586 requests for Transit permits, please reference the following:

Guidance for Submitting a PPQ 586 Permit Application (2.22 MB)

Watch our PPQ 586 "How To" videos on YouTube

PPQ 587

For guidance on PPQ 587 requests for Plants and Plant Products permits, please reference the following:

Apply for PPQ 587 Import Permits (3.77 MB)
Finding Amended or Renewed PPQ 587 Permits (536.9 KB)
How to Transfer Your PPQ Permit (510.57 KB)

Watch our PPQ 587 “How to” videos on YouTube

PPQ 588

For guidance on PPQ 588 requests for Controlled Import permits (CIP), please reference the following:

Guidance for Submitting a PPQ 588 Permit Application (2.97 MB)
Requesting Release of PPQ 588 Plant Material (482.82 KB)

Watch our PPQ 588 “How To” videos on YouTube

PPQ 621

For guidance on PPQ 621 requests for Protected Plants permits, please reference the following:

Guidance for Submitting a PPQ 621 Permit Application (2.08 MB)

Watch our PPQ 621 "How to" videos on Youtube

Veterinary Services

Watch our VS "How to" videos on YouTube

Veterinary Services Permitting Assistant

For guidance on using the VS Permitting Assistant (VSPA), please reference the following:

Using the VS Permitting Assistant (981.61 KB)

VS 16-3, VS 16-7, and VS 16-79

For guidance on VS 16-3 and 16-7 requests for Animal Products, Organisms and Vectors 16-6A permits, please reference the following:

VS 17-129

For guidance on VS 17-129 requests for Live Animal 17-135 permits, please reference the following:

Guidance for Submitting a VS 17-129 Permit Application (2.34 MB)