Skip to main content

U.S. flag An official website of the United States government

Animal and Plant Health Inspection Service
U.S. Department of Agriculture
USDA FAQ's and resources about coronavirus (COVID-19).  LEARN MORE

Potentially Actionable Suspect Sample Policy: Phyllosticta citricarpa causative agent for Citrus Black Spot (CBS)

Overview

A Potentially Actionable Suspect Sample (PASS) is a regulatory sample3 from the environment or an APHIS approved exclusionary facility5 where preliminary diagnostics indicate Phyllosticta citricarpa, a USDA regulated pathogen, is present and requires confirmation by the USDA-APHIS-PPQ-S&T, Plant Pathogen Confirmatory Diagnostics Laboratory (PPDCL).

Samples Collected from a Non-Regulated Area

Any regulatory sample collected from a non-regulated area1 or APHIS approved exclusionary facility that tests positive using APHIS approved tests for the presence of Phyllosticta citricarpa, the causative agent for Citrus black spot (CBS), is considered a PASS and must be forwarded to the USDA APHIS PPQ S&T PPCDL for final determination. Sample4 should consist of symptomatic plant material (fruit or leaves).

Sample Diagnostics
USDA APHIS PPQ Science & Technology
Plant Pathogen Confirmatory Diagnostics Laboratory (PPCDL)
9901 Powder Mill Rd. Bldg. 580
Laurel, MD 20708
Phone: (301) 313-9208 or -9271

When forwarding materials notify the lab at: APHIS-PPQCPHSTBeltsvilleSampleDiagnostics@usda.gov. You must include number of samples, screening diagnostic results and tracking information in this communication. Do not ship samples on Fridays or the day before a federal holiday.

Additional information for preparation and submission of samples can be found at Request Official Confirmation of Preliminary Pest Identifications of Domestic Samples

Samples Collected from a Regulated Area

A regulatory sample collected in a regulated area2 where preliminary diagnostics indicate Phyllosticta citricarpa is considered a PASS and requires confirmation by the USDA APHIS PPQ S&T PPCDL laboratory ONLY under the following conditions:

  • The sample is an unusual detection (novel symptoms or new host) and/or
  • The sample will result in the expansion of a previously established regulated area

If none of these conditions apply, the sample should be evaluated by the State designated laboratory for final determination.

Definitions

1 Non-Regulated Area: Any area where CBS is not known to occur in the United States.

2 Regulated Area: Any APHIS recognized area where CBS has been federally confirmed.

3 Regulatory Sample: This is a sample of regulatory concern to APHIS for CBS collected by regulatory officials.

4 Sample: A sample refers to a fruit or single bag of leaves (5-12 symptomatic leaves). It is strongly encouraged that each sample (fruit or leaf) is from a single plant.

5 APHIS approved exclusionary facility: a greenhouse structure designed to exclude quarantine pests that is approved by APHIS to produce citrus nursery stock for interstate movement.


Complementary Content
${loading}