|
TABLE OF SPECIMEN COLLECTION
| SPECIES |
TISSUES FOR MICROBIOLOGICAL AND HISTOLOGICAL
EXAMINATION |
BLOOD
SAMPLES |
OTHER |
| Bovine |
Skin and nasal swabs, prescapular lymph node (LN), body cavity fluids, joint fluids, liver, kidney,
mesenteric LN, lung, heart, tracheal swab, 3" tied-off
section of small intestine and ileum (affected area if present),
1/2 brain, any specific lesion. See Figure 1. for
brain collection from BSE suspects. |
Serum,
10 ml.
Whole blood, 20 ml. EDTA. 6 blood smears - air
dry, fix in methanol. |
External
parasites (alcohol) |
| Porcine |
Skin
swab, fluid from any affected joint, body cavity fluids, spleen, liver,
kidney, gastrohepatic and mesenteric LN, lung, tonsil, 3"
tied-off loop of small intestine and colon, 1/2 brain, any specific
lesion. |
Serum,
10 ml. Whole blood, 20 ml, EDTA. 6 blood smears - air
dry, fix in methanol. |
External
parasites (alcohol) |
| Equine |
Prescapular
LN, mandibular LN, body cavity fluids, spleen, liver, kidney,
mesenteric LN, 1/2 brain, any specific lesion. Swabs - if contagious
equine metritis suspected.* Mares - cervical, urethral,
clitoral. Stallions - penile sheath, urethral fossa, urethra. |
Serum,
20 ml. Whole blood, 20 ml, heparinized. 6 blood smears - air
dry, fix in methanol. |
External
parasites (alcohol) |
| Ovine
or Caprine |
Skin
and nasal swab, prescapular LN, mammary tissue, body cavity
fluids, spleen, liver, kidney, mesenteric LN, lung, pleural
LN, tracheal and bronchial swabs, 1/2 brain, any specific lesion. |
Serum,
10 ml. Whole blood, 10 ml, heparinized. 6 blood smears
- air dry, fix in methanol. |
External
parasites (alcohol) |
| Avian |
Tracheal
and cloacal swabs, liver, spleen, kidney, lung, trachea, bone
marrow, heart, ovary, brain, terminal intestine, bursa of Fabricius,
any specific lesion. |
Serum,
2 ml. |
External
parasites (alcohol) Tracheal and nasal swab |
| Vesicular |
Vesicular
fluid (all that is obtainable), vesicular lesion epithelium,
flaps of epithelial tissue, esophageal-pharyngeal fluid (10
ml before dilution with tris-buffered tryptose broth, pH 7.4-7.6
with phenol red). In addition, if dead--prescapular LN, adrenal,
kidney, thyroid, heart, tonsil, mandibular LN. |
Serum,
10 ml. |
|
*Amies
transport medium with charcoal required; specimens must reach the
NVSL within 48 hours after collection.
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