USDA - APHIS - Plant Health, Plant Protection and Quarantine

Phytophthora ramorum/ Sudden Oak Death

Protocols

Diagnostic

Diagnostics Work Instruction Table (PDF; 99 Kb)

For the latest version of the P. ramorum diagnostic protocols, please contact Renee DeVries at: Renee.M.DeVries@aphis.usda.gov

Sampling

Potentially Actionable Suspect Sample (PASS) System (revised 2/11/12) (PDF; 110 Kb)
Soil and Container Mix Protocol (PDF; 260 Kb)
Water Sampling Protocol - Appendix to the Nursery Survey Manual

Diagnostic Laboratory List (PDF; 24 Kb) - list of diagnostics laboratories accredited under the NPPLAP program that have passed proficiency test in molecular diagnostics for P. ramorum. Confirmatory tests have to performed at the APHIS-PPQ, Molecular Diagnostics Laboratory, in Beltsville, MD.

Response

Confirmed Nursery Protocol (Wholesale and Production Nurseries) (Revised 2/15/13) (PDF; 1.70 Mb)
Retail Nursery Protocol (Revised 2/15/13) (PDF; 1.64 Mb)
Residential and Landscaped Commercial Settings Protocol (Revised 2/15/13) (PDF; 174 Kb)
Trace Back Protocol v. 1.3 (10/6/09) (PDF; 262 Kb)
Trace Forward Protocol v. 2.1 (Modified 6/25/10) (PDF; 312 Kb)