Plant Health
Phytophthora ramorum/ Sudden Oak Death |
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Protocols
Diagnostic
- Culture/Morphology Protocol (revised 6/8/07) (PDF; 240 Kb)
- Diagnostics Summary (revised 6/8/07) (PDF; 47 Kb)
- Diagnostics Schematic (PDF; 12 Kb)
- Elicitin Real Time PCR Protocol (revised 6/8/2008) (PDF; 382 Kb)
- ELISA Protocol (PDF; 15 Kb)
- ITS Real Time PCR Protocol (revised 6/4/2007) (PDF; 234 Kb)
- Nested PCR Protocol (revised 6/4/07) (PDF; 108 Kb)
*Flow chart for using Elicitin RT PCR results for regulatory determinations is pending.
For the latest version of the P. ramorum diagnostic protocols, please contact Renee DeVries at: Renee.M.DeVries@aphis.usda.gov
Sampling
- Potentially Actionable Suspect Sample (PASS) System (revised 3/28/08) (PDF; 48 Kb)
- Soil and Growing Medium Sampling and Testing Protocol - Appendix to the Confirmed Nursery Protocol (4/22/08) (PDF; 504 Kb)
- Water Sampling Protocol - Appendix to the Confirmed Nursery Protocol (revised 4/07) (PDF; 43 Kb)
Diagnostic Laboratory List (PDF; 24 Kb) - list of diagnostics laboratories accredited under the NPPLAP program that have passed proficiency test in molecular diagnostics for P. ramorum. Confirmatory tests have to performed at the APHIS-PPQ, Molecular Diagnostics Laboratory, in Beltsville, MD.
Survey
- Nursery Survey Manual (3/16/07 - updated 1/23/2008)
Response
- Confirmed Nursery Protocol Version 8.0 (7/20/07 - Updated October 28, 2008) (PDF; 483 Kb)
- Residential and Landscaped Commercial Settings Protocol (9/1/09) (PDF; 170 Kb)
- Retail Nursery Protocol, Version 1 (Modified 8/12/09) (PDF; 434 Kb)
- Trace Back Protocol v. 1.3 (10/6/09) (PDF; 262 Kb)
- Trace Forward Protocol v. 2.1 - Revised (6/5/08) (PDF; 215 Kb)