Plant Health
Phytophthora ramorum/ Sudden Oak Death |
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Protocols
Diagnostic
- Diagnostics Summary (revised 6/8/07) (PDF; 47 Kb)
- Diagnostics Schematic (PDF; 12 Kb)
- ELISA Protocol (PDF; 15 Kb)
- Culture/Morphology Protocol (revised 6/8/07) (PDF; 240 Kb)
- Nested PCR Protocol (revised 6/4/07) (PDF; 108 Kb)
- ITS Real Time PCR Protocol (revised 6/4/2007) (PDF; 234 Kb)
- Elicitin Real Time PCR Protocol (revised 6/8/2008) (PDF; 382 Kb)
*Flow chart for using Elicitin RT PCR results for regulatory determinations is pending.
For the latest version of the P. ramorum diagnostic protocols, please contact Renee DeVries at: Renee.M.DeVries@aphis.usda.gov
Sampling
- Potentially Actionable Suspect Sample (PASS) System (revised 3/28/08) (PDF; 48 Kb)
- Soil and Growing Medium Sampling and Testing Protocol - Appendix to the Confirmed Nursery Protocol (4/22/08) (PDF; 504 Kb)
- Water Sampling Protocol - Appendix to the Confirmed Nursery Protocol (revised 4/07) (PDF; 43 Kb)
Survey
- Nursery Survey Manual (3/16/07 - updated 1/23/2008)
Response
- Confirmed Nursery Protocol Version 8.1 (6/5/08)
- Rationale and Highlights of Changes in CNP Version 8.1 (6/5/08)
[Above documents are unavailable and under review. Use the Confirmed Nursery Protocol Version 8 dated 7/20/07 (link below) until further notice. Contact JJones at 301.734.5038 if more information is needed.]
For Reference:
Confirmed Nursery Protocol Version 8 (7/20/07 - Updated June 26, 2008) (PDF; 425 Kb)
- Forest Wildland Protocol (6/16/06) (PDF; 355 Kb)
- Retail Nursery Eradication Protocol, Version 1 (12/19/07) (PDF; 327 Kb)
- Residential and Commercial Sites Eradication Protocol (12/20/07) (PDF; 61 Kb)
- Trace Back Protocol v. 1.2 (2/14/08) (PDF; 211 Kb)
- Revised Trace Forward Protocol v. 2.1 (6/5/08) (PDF; 215 Kb)
CPHST P. ramorum Diagnostic Provisional Approval Documents
- Provisional Approval Process Introduction Letter (PDF; 95 Kb)
- Laboratory Guide (PDF; 158 Kb)
- Provisional Approval Inspection Checklist (PDF; 27 Kb)
- Provisional Approval Proficiency Program Letter (PDF; 122 Kb)
- Provisionally Approved Labs and Diagnostician List for 2007 (PDF; 16 Kb)