Table of Specimen Collection
SPECIES |
TISSUES FOR MICROBIOLOGICAL AND HISTOLOGICAL EXAMINATION |
BLOOD SAMPLES |
OTHER |
Bovine |
Skin and nasal swabs, prescapular lymph node (LN), body cavity fluids, joint fluids, liver, kidney, mesenteric LN, lung, heart, tracheal swab, 3" tied-off section of small intestine and ileum (affected area if present), 1/2 brain, any specific lesion. |
Serum, 10 ml.
Whole blood, 20 ml. EDTA. 6 blood smears - air dry, fix in methanol. |
External parasites (alcohol) |
Porcine |
Skin swab, fluid from any affected joint, body cavity fluids, spleen, liver, kidney, gastrohepatic and mesenteric LN, lung, tonsil, 3" tied-off loop of small intestine and colon, 1/2 brain, any specific lesion. |
Serum, 10 ml. Whole blood, 20 ml, EDTA. 6 blood smears - air dry, fix in methanol. |
External parasites (alcohol) |
Equine |
Prescapular LN, mandibular LN, body cavity fluids, spleen, liver, kidney, mesenteric LN, 1/2 brain, any specific lesion. Swabs - if contagious equine metritis suspected.* Mares - cervical, urethral, clitoral. Stallions - penile sheath, urethral fossa, urethra. |
Serum, 20 ml. Whole blood, 20 ml, heparinized. 6 blood smears - air dry, fix in methanol. |
External parasites (alcohol) |
Ovine or Caprine |
Skin and nasal swab, prescapular LN, mammary tissue, body cavity fluids, spleen, liver, kidney, mesenteric LN, lung, pleural LN, tracheal and bronchial swabs, 1/2 brain, any specific lesion. |
Serum, 10 ml. Whole blood, 10 ml, heparinized. 6 blood smears - air dry, fix in methanol. |
External parasites (alcohol) |
Avian |
Tracheal and cloacal swabs, liver, spleen, kidney, lung, trachea, bone marrow, heart, ovary, brain, terminal intestine, bursa of Fabricius, any specific lesion. |
Serum, 2 ml. |
External parasites (alcohol) Tracheal and nasal swab |
Vesicular |
Vesicular fluid (all that is obtainable), vesicular lesion epithelium, flaps of epithelial tissue, esophageal-pharyngeal fluid (10 ml before dilution with tris-buffered tryptose broth, pH 7.4-7.6 with phenol red). In addition, if dead--prescapular LN, adrenal, kidney, thyroid, heart, tonsil, mandibular LN. |
Serum, 10 ml. |
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*Amies transport medium with charcoal required; specimens must reach the NVSL within 48 hours after collection. |