Scrapie Genetic Susceptibility Testing
Genetic Resistance to Scrapie (pdf 16kb) - Basic information on genetics and scrapie resistance and information on how APHIS is using genetic testing to help eradicate scrapie.
Genotype testing for susceptibility to scrapie is a key element of the Scrapie Eradication Program. Consequently, the Animal and Plant Health Inspection Service (APHIS) has approved the following companies to perform sheep genotyping for the prion protein gene at codons 136, 154, and 171 in an official capacity for the National Scrapie Eradication Program (NSEP). This approval is in accordance with Title 9, Code of Federal Regulations, Part 54.11 and Veterinary Services Memorandum Number 557.6.
The approved laboratories are eligible to conduct privately funded official scrapie genotype testing and compete for NSEP funded official genotyping. This approval lasts for 1 year and is subject to review and renewal.
Each laboratory applied for approval to conduct official genotype tests. The approval process begins by the laboratory sending an application package to the National Veterinary Services Laboratory (NVSL). The application must contain the following information about the laboratory:
Following review of the application package for completeness, each laboratory is required to complete a preliminary test panel of samples to demonstrate basic technical competency for the required tests. NVSL then schedules and conducts a site visit to meet laboratory personnel, observe laboratory operations, inspect facilities and evaluate testing and laboratory protocols. Laboratory that successfully passes this site visit is then sent a second proficiency test panel to demonstrate a statistically high level of accuracy in conducting the genotyping tests. Following successful completion of the final proficiency panel the laboratory is given written notification of APHIS approval to conduct official scrapie genotype testing.
If you have any questions or are a representative of a laboratory that would like to be considered for approval to participate in the genotyping of sheep for the Scrapie Eradication Program, please contact Diane.L.Sutton@aphis.usda.gov.
Last Modified: July 27, 2011